Mitomycin c prophage

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WebMitomycin C has also been used topically rather than intravenously in several areas. The first is cancers, particularly bladder cancers and intraperitoneal tumours. It is now well … WebInduction of phage formation in the lysogenic Escherichia coli K-12 by mitomycin C Nature. 1959 Oct 3;184(Suppl 14):1079-80. doi: 10.1038/1841079b0. Authors N OTSUJI, M SEKIGUCHI, T IIJIMA, Y TAKAGI. PMID: 14429381 DOI: 10.1038/1841079b0 No abstract available. MeSH terms ...

Phage release from biofilm and planktonic - Oxford Academic

WebMitomycin C (215a) shows a potent activity against a wide spectrum of tumors and bacteria. In spite of its high toxicity it is extensively used in chemotherapy especially in treating gastrointestinal cancers 〈B-80MI 801-01〉. For synthetic chemists the structure of mitomycins presents a formidable challenge. david bowie real name 1

Effect of mitomycin C on the synthesis of bacterial and viral ...

Web12 mei 2024 · To explore this possibility, we first tested the inducibility of prophages by the antibiotic mitomycin C in five human isolates of Staphylococcus aureus using a plaque … Web1 nov. 2005 · Mitomycin C induced phage release in 48-h biofilm cultures only about 10-fold. 5FU did slightly increase the phage-release about 10-fold in the 6-h biofilm samples, however, it did not induce phage release in 6-h planktonic or 48-h biofilm cultures ... This causes prophage activation, which leads to phage release by cell lysis . Web6 dec. 2024 · Mitomycin C acts on DNA in three possible ways: promoting (a) cross-linking events, (b) alkylation and not necessarily cross-linking, and (c) strand breakage. … david bowie relatives

Detecting Lactococcus lactis Prophages by Mitomycin C …

Survival, DNA-breakdown and induction of prophage lambda in a ...

WebTo understand the function of these prophages, cultures of TM1040 were treated with mitomycin C to induce the production of viral particles. A significant increase in viral counts and a decrease in bacterial counts when treated with mitomycin C suggested that prophages were induced from TM1040. WebMitomycin C-treated bacteria resumed DNA synthesis also when infected with either T 3 or T 5 phage. 3. 3. Although mitomycin C did not affect the infectivity of free phage, the reproduction of phage was progressively impaired with increasing concentrations of the antibiotic. Phage-infected bacteria in contact with high levels of mitomycin C ... david bowie record salesWebDNA synthesis is selectively inhibited in Salmonella typhimurium by treatment with the antibiotic mitomycin C. These inhibited cells will, however, produce active particles on infection with phage P22. Genetic recombination between marked phages is not affected, but the frequency of lysogenization is drastically reduced under these conditions. david bowie record store day releases

"WebMitomycin C and UV-C are commonly used to in- duce lysogens (Ackermann & DuBow 1987). Based on studies in the phage lambda (Gottesman & Oppenheim 1994), … " - Mitomycin c prophage

Mitomycin c prophage

Web16 jun. 2024 · Gene Map and Features of Φstx 2dact prophage. Lambdoid phage Φ1720a-02 was originally isolated from EC1720a-02, a STEC strain found in packaged ground beef [].To create the novel C. rodentium-mediated mouse model of EHEC infection, C. rodentium DBS100 (also known as C. rodentium strain ICC 168 (GenBank accession number … WebVibrio vulnificus is a bacterium known to cause fatal necrotizing soft tissue infection in humans. Here, a remarkable therapeutic effect of hyperbaric oxygen (HBO) on V. vulnificus infection provoked by its injection into mouse footpads is described. HBO was shown to be bactericidal to this bacterium in vitro as well as in the infected tissue. The bactericidal …

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WebThe mitomycins are antitumor antibiotics that are under investigation now for more than 30 years. Mitomycin C (MMC) is the best investigated subtype. It serves as a prototype for drugs with bioreductive alkylation, which is a unique feature of this class. MMC is mainly active under anaerobic circumstances. Web19 jul. 2024 · Detecting Lactococcus lactis Prophages by Mitomycin C-Mediated Induction Coupled to Flow Cytometry Analysis Detecting Lactococcus lactis Prophages by Mitomycin C-Mediated Induction Coupled to Flow Cytometry Analysis Front Microbiol. 2024 Jul 19;8:1343. doi: 10.3389/fmicb.2024.01343. eCollection 2024. Authors

WebProphage Induction Causes Geobacter Electroactive Biofilm Decay. Liu, Ye, Zhang, Rensing, Zhou, Nealson ... and a mitomycin C induction assay revealed the lysogenic to lytic transition of those prophages, resulting in a progressive decay in both current generation and the EAB. WebExposure to mitomycin C induces no DNA breakdown in these mutants (Boyce & Howard-Flanders, 1964). A recA uvr A double mutant was first constructed by Howard-Flander & Boycse (1966). The ultraviolet dose for 37 % survival of the double mutant is 0-2 erg/mm2, corresponding approximately to one dimer per bacterial genome (Howard-Flanders, 1968).

Web19 sep. 2016 · Although activation of the C. pasteurianum prophage occurred spontaneously in the aforementioned analysis, prophage excision can be artificially induced using ultraviolet irradiation or mitomycin ... Web24 aug. 2024 · When the culture reached an OD 600nm of 0.15–0.25, mitomycin C was added to 0.3 μg/ml, inverted several times for gentle mixing, and incubated at 37 °C in an anaerobic chamber for 15–20 h.

Web1 dec. 1995 · A prophage was induced from cells of the pathogenic spirochaete Serpulina hyodysenteriae using mitomycin C. Five to seven hours after mitomycin C was added …

Web19 nov. 2014 · In response to mitomycin C induction, virus-like particles can increase to 7 × 109 per ml, while host cells decrease an order of magnitude. The vB_CibM-P1 … david bowie red shoes danceWeb5 aug. 2003 · Parasite adaptation to dynamic host characteristics is a common theme in biology. We recently identified a unique mechanism of adaptation that governs the interactions between a group of bacterial pathogens belonging to the Bordetella genus and a family of bacteriophages that infect them ().As pathogens of numerous mammalian … david bowie record store dayWeb24 sep. 2024 · Genome analysis of representative strains of the 7 genetic lineages of Lactococcus lactis constituting the culture indicated the presence of prophages in … gas grill char-broilWebMost bacterial cells carry prophage genomes either integrated into the host DNA or present as repressed plasmids. Methods are described for the induction of prophages using … david bowie repetitionWebThe genome of strain MGAS315 has six prophage-like elements, but it is unknown if they can be induced. Mitomycin C is a DNA-damaging agent and is known to activate recA … david bowie relationshipsWeb21 dec. 2024 · In contrast, in the presence of mitomycin C, the fitness benefits of ARGs relative to prophages were less pronounced or even absent, and lysogen fitness was primarily determined by prophage carriage (in Cluster 1 there was a higher benefit of prophages than ARGs, and mitomycin C was present in most of these treatment … gas grill charcoal comboWeb16 aug. 2024 · For prophage induction experiments, mitomycin C (1 mg/mL) was added 1 h after cell recovery from the electroporation. Bacteria were then plated at the … david bowie releases 2022