WebMay 10, 2024 · Results: In this study, a conserved segment containing one SNP A4408C of parvovirus was used for real-time PCR amplification. Subsequently, data were auto-analyzed and plotted using Applied Biosystems® High Resolution Melt Software v3.1. Results showed that CPV and FPV can be detected simultaneously in a single PCR reaction. WebIn this study, according to phenotypic methods, gentamicin and ciprofloxacin-resistant strains had the highest frequency with 95.04% and 96.03%, respectively, and amikacin (6.66%) and colistin (3.96%) showed the lowest abundance. Moreover, MDR and XDR strains were counted as 38.61% and 7.92% of isolates, respectively.
Prevalence and Molecular Typing of Colistin-Resistant
WebHigh Resolution Melt Software v3.1 is an all-new and improved tool for High Resolution Melting (HRM) analysis. Shop Applied Biosystems™ High Resolution Melt … WebSep 12, 2013 · High resolution melting analysis (HRM) is a rapid and cost-effective technique for the characterisation of PCR amplicons. ... The analyses of the HRM data were performed using the Light Cycler Software (Version 1.5) and the Eco™ Software (v3.0.16.0), respectively (fluorescence signal normalisation is implemented in the software). grafton cycle car
Melting curve analysis software ResearchGate
WebApr 3, 2024 · Because the melting peak showed complete melting of the amplicon at 85°C ( Figure 2 .G.2), we investigated if it was indeed possible to specifically detect the FAM signal, in the presence of SYBR green, at a temperature higher than the melting temperature of the amplicon (i.e. 85°C). Web2. FEATURES in HRM v3.0.1 The HRM v3.0.1 Software contains all features from the previous version 3.0 as well as the following: • New feature added a) HRM v3.0.1 Software … WebJun 15, 2024 · Melting curve plots were generated and analysed using High Resolution Melt software v3.0.1 (Applied Biosystems) to determine average melting temperature (T m) for each Leptospira spp.... grafton cycles